Indian Muntjac Nuclei
UV-2A Longpass Emission (Wide Bandwidth Excitation) Ultraviolet Set
Fluorescence emission intensity from a culture of Indian Muntjac deerskin fibroblasts stained with Hoechst 33258, which specifically binds to DNA and stains the nuclei. The ultraviolet absorption maximum of Hoechst 33258 is 346 nanometers and the emission maximum is 460 nanometers. In addition, the specimen was also stained with Alexa Fluor 568 phalloidin (targeting actin; excitation at 578 nanometers and red emission at 600 nanometers). Note the presence of signal from the red-stained actin filaments.