Localizing Fluorescent Tags to the Golgi Complex and the F-Actin Cytoskeleton in Rat Stomach Samples
This widefield image of a rat stomach tissue section was produced by probing the specimen with Alexa Fluor 488, Texas Red, and Hoechst 33342. The Alexa Fluor dye was conjugated to phalloidin, targeting the cytoskeletal F-actin network, and Texas Red was conjugated to WGA in order to localize a red fluorescent tag to the Golgi complex. The nuclear counterstain Hoechst 33342 was employed to visualize cell nuclei. Images were recorded in grayscale with a 12-bit digital camera coupled to a Nikon Eclipse 80i microscope equipped with bandpass emission fluorescence filter optical blocks. During the processing stage, individual image channels were pseudocolored with RGB values corresponding to each of the fluorophore emission spectral profiles.