Mouse Intestine Thin Section

BV-2B Longpass Emission (Medium Bandwidth Excitation) Blue-Violet Set

Fluorescence emission intensity from a thin section of mouse intestine stained with Alexa Fluor 350 wheat germ agglutinin, a blue fluorescent lectin that is specific to the mucus of goblet cells. The ultraviolet absorption maximum of Alexa Fluor 350 is 346 nanometers and the emission maximum is 442 nanometers. In addition, the specimen was simultaneously stained with Alexa Fluor 568 phalloidin (filamentous actin; 600 nanometer emission) and SYTOX Green (nuclei; 523 nanometer emission). Note the presence of high signal levels from the green fluorophore, but the absence of fluorescence from Alexa Fluor 350 (blue emission), which is not efficiently excited nor detected by the BV-2B filter combination. In many cases, as is evident in the image, SYTOX Green stains other cytoplasmic components in addition to DNA. Signal from the red fluorophore (Alexa Fluor 568) is also not observed in this specimen.

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