Mouse Intestine Thin Section

B-1A Longpass Emission (Narrow Bandwidth Excitation) Blue Set

Fluorescence emission intensity from a thin section of mouse intestine stained with Alexa Fluor 350 wheat germ agglutinin, a blue fluorescent lectin that is specific to the mucus of goblet cells. The visible light absorption maximum of Alexa Fluor 350 is 346 nanometers and the emission maximum is 442 nanometers, which hinders the ability to image the dye with blue excitation filter combinations. In addition, the specimen was simultaneously stained with Alexa Fluor 568 phalloidin (filamentous actin; 600 nanometer emission) and SYTOX Green (nuclei; 504 nanometer excitation and 523 nanometer emission). Note the low level of background noise in comparison to the other Nikon longpass filter combinations (B-2A andB-3A), and the significant amount of red signal arising from Alexa Fluor 568 that appears in the image.