Mouse Intestine Thin Section

DAPI-FITC Bandpass Emission (Dual Band Excitation) Set

Fluorescence emission intensity from a thin section of mouse intestine stained with Alexa Fluor 350 wheat germ agglutinin, a blue fluorescent lectin that is specific to the mucus of goblet cells. In addition, the specimen was simultaneously stained with Alexa Fluor 568 phalloidin (filamentous actin; 600 nanometer emission) and SYTOX Green (nuclei; 504 nanometer excitation and 523 nanometer emission). Note the low signal level from the blue fluorophore (Alexa Fluor 350), but the bright green fluorescence of nuclei in the tissue specimen due to SYTOX Green fluorescence. This filter combination effectively eliminates signal from the red (Alexa Fluor 568) probe.