Mouse Intestine Thin Section

Y-2E/C Bandpass Emission (Medium Bandwidth Excitation) Yellow Set

Fluorescence emission intensity from a thin section of mouse intestine stained with Alexa Fluor 350 wheat germ agglutinin, a blue fluorescent lectin that is specific to the mucus of goblet cells. In addition, the specimen was simultaneously stained with Alexa Fluor 568 phalloidin (filamentous actin; 600 nanometer emission) and SYTOX Green (nuclei; 504 nanometer excitation and 523 nanometer emission). Note the significant amount of orange-red signal arising from Alexa Fluor 568 that has stained the actin network in the brush border.