Mouse Intestine Thin Section

G-2A Longpass Emission (Wide Bandwidth Excitation) Green Set

Fluorescence emission intensity from a thin section of mouse intestine stained with Alexa Fluor 350 wheat germ agglutinin, a blue fluorescent lectin that is specific to the mucus of goblet cells. In addition, the specimen was simultaneously stained with Alexa Fluor 568 phalloidin (filamentous actin; 600 nanometer emission) and SYTOX Green (nuclei; 504 nanometer excitation and 523 nanometer emission). Note the higher level of background noise in comparison to the Nikon bandpass filter combinations (G-2E/C and Cy3), and the significant amount of orange-red signal arising from Alexa Fluor 568 that appears in the image.