Mouse Kidney Tissue
B-2A Longpass (Medium Bandwidth Excitation) Blue Set
Fluorescence emission intensity from a thin section of mouse kidney labeled with multiple (3) fluorophores. Nuclei in the tissue section were targeted with the nucleic acid probe DAPI, which has an excitation maximum at 358 nanometers and an emission maximum at 461 nanometers when bound to DNA in cell cultures and tissue sections. In addition, the cryostat section was also simultaneously stained with Alexa Fluor 488 wheat germ agglutinin (glomeruli and convoluted tubules) and Alexa Fluor 568 phalloidin (filamentous actin and the brush border). Note the absence of signal from the blue (DAPI) fluorophore but the low level of red (Alexa Fluor 568) fluorescence bleed-through in many regions of the image.