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Pig Kidney Cytokeratin Intermediate Filaments with Cy5

Cy5 HYQ Bandpass Emission (Wide Bandwidth Excitation) Red Set

Fluorescence emission intensity from a culture of male pig kidney cells (Sus scrofaLLC-PK1 line) that were immunofluorescently labeled with primary anti-cytokeratin (pan) mouse monoclonal antibodies followed by goat anti-mouse Fab fragments conjugated to Cy5. The visible light absorption maximum of Cy5 is 649 nanometers and the emission maximum occurs at 670 nanometers. The image was captured in 12-bit grayscale mode, but subsequently pseudocolored by converting to the RGB color palette and setting the green and blue channels to zero (to produce a red image). Note the bright fluorescence intensity exhibited by the intermediate filament network, which extends throughout the cytoplasm in these epithelial cells.

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