Potato Starch Grains
G-2B Longpass Emission (Wide Bandwidth Excitation) Green Set
Autofluorescence emission intensity from starch grains in a thin section of potato (Solanum tuberosum) tuber tissue. Endogenous autofluorescence in plant tissues arises from a variety of biomolecules, including chlorophyll, carotene, and xanthophyll. In the green excitation region, chlorophyll has an absorption band with a low extinction coefficient, but still produces a detectable level of fluorescence at emission wavelengths of 550 nanometers and above (green, yellow, orange, and red). For the starch grains illustrated above, note the presence of autofluorescence emission intensity in the red spectral region.