Potato Tissue Section
Cy5 HYQ Bandpass Emission (Wide Bandwidth Excitation) Red Set
Autofluorescence emission intensity from starch grains and other components in a thin section of potato (Solanum tuberosum) tuber tissue. Endogenous autofluorescence in plant tissues arises from a variety of biomolecules, including chlorophyll, carotene, and xanthophyll. In the red absorption region, chlorophyll has an absorption band with a low extinction coefficient, but still produces a detectable level of fluorescence at emission wavelengths of 650 nanometers and above (deep red region). For the starch grains illustrated above, note the presence of autofluorescence emission intensity in the red spectral region.