Rabbit Bone Marrow Tissue with DAPI, Oregon Green 488, and Alexa Fluor 546
DAPI-FITC-Texas Red Bandpass Emission (Triple Band Excitation) Set
Fluorescence emission intensity from a thin section of rabbit bone marrow tissue stained for DNA and glycoproteins with DAPI and Oregon Green 488 conjugated to wheat germ agglutinin, respectively. The ultraviolet light absorption maximum of DAPI is 358 nanometers and the visible emission maximum occurs at 461 nanometers, while the corresponding values for Oregon Green are 496 and 524 nanometers. In addition, the specimen was simultaneously labeled with Alexa Fluor 546 conjugated to phalloidin (targeting the actin network; yellow-orange emission). Note the presence of both overlapping and discrete signals from all three of these fluorophores.