Rat Kangaroo Cell DNA and Tubulin

DAPI-FITC Bandpass Emission (Dual Band Excitation) Set

Fluorescence emission intensity from a culture of rat kangaroo (PtK2) epithelial cells that were immunofluorescently labeled with primary anti-bovine alpha-tubulin mouse monoclonal antibodies followed by goat anti-mouse Fab fragments conjugated to Alexa Fluor 488. The visible light absorption maximum of Alexa Fluor 488 is 495 nanometers and the emission maximum occurs at 519 nanometers (in the green region of the visible light spectrum). In addition, the specimen was labeled with Hoechst 33258, which selectively binds to DNA in the cell nucleus, and MitoTracker Red CMXRos (targeting mitochondria; red fluorescence). Note the prominent green staining of the intracellular microtubule network that extends throughout the cytoplasm, and the blue emission from Hoechst 33258 bound to DNA in the nucleus. The cell in the lower portion of the image appears to be undergoing mitosis in the prometaphase or metaphase state.

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