Rat Kangaroo Cell Intermediate Filaments with Cy2
YFP Bandpass Emission (Narrow Bandwidth Excitation) Blue-Green Set
Fluorescence emission intensity from a culture of rat kangaroo kidney (PtK2 line) epithelial cells that were immunofluorescently labeled with primary anti-keratin (an intermediate filament protein) mouse monoclonal antibodies followed by goat anti-mouse Fab fragments conjugated to the cyanine dye, Cy2. In addition, the specimen was simultaneously stained for DNA with the ultraviolet-absorbing probe DAPI, and for the intracellular mitochondrial network with MitoTracker Red CMXRos. The visible light absorption maximum of Cy2 is 489 nanometers and the emission maximum occurs at 506 nanometers, values that fall within the useful range of the Nikon YFP HYQ filter combination. Note the high level of green fluorescence observed from the keratin intermediate filament network that extends throughout the cytoplasm, but an absence of signal from the blue and red fluorophores (DAPI and MitoTracker) targeting the nuclei and mitochondria, respectively.