Rat Kangaroo Cell Mitotic Spindle
BV-2B Longpass Emission (Medium Bandwidth Excitation) Blue-Violet Set
Fluorescence emission intensity from a culture of rat kangaroo (PtK2) epithelial cells that were immunofluorescently labeled with primary anti-bovine alpha-tubulin mouse monoclonal antibodies followed by goat anti-mouse Fab fragments conjugated to Alexa Fluor 488. Note the prominent staining of the intracellular microtubule network that extends throughout the cytoplasm. In addition, the specimen was simultaneously stained for the nuclear protein cdc6 (conjugated to Pacific Blue), and for F-actin with phalloidin conjugated to Alexa Fluor 568. Note the absence of signal from the red (Alexa Fluor 568) fluorophore, but the presence of significant fluorescence emission from the blue (Pacific Blue) and green (Alexa Fluor 488) probes, which clearly define the mitotic spindle apparatus in this dividing cell.