Rat Kangaroo Cell Tubulin
G-2B Longpass Emission (Wide Bandwidth Excitation) Green Set
Fluorescence emission intensity from a culture of rat kangaroo (PtK2) epithelial cells that were immunofluorescently labeled with primary anti-bovine alpha-tubulin mouse monoclonal antibodies followed by goat anti-mouse Fab fragments conjugated to Alexa Fluor 546. The visible light absorption maximum of Alexa Fluor 546 is 556 nanometers and the emission maximum occurs at 573 nanometers (in the yellow region of the visible light spectrum). Note the prominent red staining of the intracellular microtubule network that extends throughout the cytoplasm of both the large cell and the daughter cells undergoing cytokinesis in the upper right-hand side of the image.