Rat Kangaroo Cytokeratin Intermediate Filaments with Alexa Fluor 647

Cy5 HYQ Bandpass Emission (Wide Bandwidth Excitation) Red Set

Fluorescence emission intensity from a culture of rat kangaroo kidney epithelial cells (PtK2line) that were immunofluorescently labeled with primary anti-cytokeratin (pan) mouse monoclonal antibodies followed by goat anti-mouse Fab fragments conjugated to Alexa Fluor 647. The visible light absorption maximum of Alexa Fluor 647 is 649 nanometers and the emission maximum occurs at 666 nanometers. The image was captured in 12-bit grayscale mode, but subsequently pseudocolored by converting to the RGB color palette and setting the green and blue channels to zero (to produce a red image). Note the bright fluorescence intensity exhibited by the intermediate filament network, which extends throughout the cytoplasm in these epithelial cells.