Rat Kangaroo Kidney Cell Vimentin

Cy5 HYQ Bandpass Emission (Wide Bandwidth Excitation) Red Set

Fluorescence emission intensity from a culture of rat kangaroo (PtK2 line) epithelial kidney cells that were immunofluorescently labeled with primary anti-vimentin mouse monoclonal antibodies followed by goat anti-mouse F(ab')2 fragments conjugated to biotin. The biotin secondary antibody was subsequently absorbed with a streptavidin conjugate of QDot 655. The visible light absorption extinction coefficient of QDot 655 occurs over a wide range that increases with decreasing wavelength. Fluorescence emission features a symmetrical profile in quantum dots (centered at 655 nanometers for this species). Images of the preparation were captured in 12-bit grayscale mode, but subsequently pseudocolored by converting to the RGB color palette and setting the green and blue channels to zero (to produce a red image). Note the bright fluorescence intensity exhibited by the vimentin intermediate filament network that extends throughout the cytoplasm.