Rat Kangaroo Kidney Cells with Cy2, MitoTracker Red CMXRos, and DAPI

DAPI-FITC-Texas Red Bandpass Emission (Triple Band Excitation) Set

Fluorescence emission intensity from a culture of rat kangaroo kidney epithelial (PtK2 line) cells that were immunofluorescently labeled with primary anti-cytokeratin mouse monoclonal antibodies followed by goat anti-mouse Fab fragments conjugated to Cy2. The visible light absorption maximum of Cy2 is 489 nanometers and the emission maximum occurs at 506 nanometers. Note the prominent staining of the cytokeratin network throughout the cytoplasm in these cells. In addition, the specimen was simultaneously stained for DNA with the ultraviolet-absorbing probe DAPI, and for the mitochondrial network with MitoTracker Red CMXRos. Keratins are water-insoluble proteins that form monofilaments, one of the many classes of intermediate filaments that comprise the cytoskeletal complex in most epithelial tissues.