Rat Kangaroo Vimentin Intermediate Filament Network with Cy5

Cy5 HYQ Bandpass Emission (Wide Bandwidth Excitation) Red Set

Fluorescence emission intensity from a culture of rat kangaroo (PtK2 line) epithelial kidney cells that were immunofluorescently labeled with primary anti-vimentin mouse monoclonal antibodies followed by goat anti-mouse Fab fragments conjugated to the cyanine dye, Cy5. The visible light absorption maximum of Cy5 is 649 nanometers and the emission maximum occurs at 670 nanometers. Images of the preparation were captured in 12-bit grayscale mode, but subsequently pseudocolored by converting to the RGB color palette and setting the green and blue channels to zero (to produce a red image). Note the bright fluorescence intensity exhibited by the fibrous vimentin intermediate filament network that extends throughout the cytoplasm.