Rat Rectum Tissue Labeled with Oregon Green 488

The rat rectum tissue section presented in the digital image above was labeled with Oregon Green 488 conjugated to the lectin wheat germ agglutinin. Fluorescent wheat germ agglutinin conjugates are often used as probes for the Golgi network in mammalian cultures and tissue samples. The tissue section was also stained with Alexa Fluor 568 conjugated to phalloidin and Hoechst 33342, which target F-actin and DNA, respectively. Images were recorded in grayscale with a 12-bit digital camera coupled to a Nikon Eclipse 80i microscope equipped with bandpass emission fluorescence filter optical blocks. During the processing stage, individual image channels were pseudocolored with RGB values corresponding to each of the fluorophore emission spectral profiles.