Rat Rectum Tissue Section Triple Stained for the Golgi Network, F-Actin, and Nuclei

A rat rectum tissue section (presented above) was stained with Oregon Green 488 conjugated to wheat germ agglutinin in order to target the Golgi apparatus. In addition, the cytoskeletal filamentous actin network was labeled with Alexa Fluor 568 conjugated to phalloidin and cell nuclei were stained with Hoechst 33342. Images were recorded in grayscale with a 12-bit digital camera coupled to a Nikon Eclipse 80i microscope equipped with bandpass emission fluorescence filter optical blocks. During the processing stage, individual image channels were pseudocolored with RGB values corresponding to each of the fluorophore emission spectral profiles.