Rat Seminal Vesicle Tissue Section

The cytoskeletal filamentous actin network was targeted in a rat seminal vesicle tissue sample with phalloidin conjugated to Alexa Fluor 568. Phalloidin is a member of the phallotoxin group of bicyclic peptides isolated from the deadly Amanita phalloides mushroom. The tissue section was also labeled for nuclear DNA with Hoechst 33342. Images were recorded in grayscale with a 12-bit digital camera coupled to a Nikon Eclipse 80i microscope equipped with bandpass emission fluorescence filter optical blocks. During the processing stage, individual image channels were pseudocolored with RGB values corresponding to each of the fluorophore emission spectral profiles.