Rat Skeletal Muscle CDC6
BV-1A Longpass Emission (Narrow Bandwidth Excitation) Blue-Violet Set
Fluorescence emission intensity from a culture of rat skeletal muscle tissue cells (L6 cell line; myoblasts) that were immunofluorescently labeled with primary anti-cdc6 (human) mouse monoclonal antibodies followed by goat anti-mouse Fab fragments conjugated to Marina Blue. The ultraviolet absorption maximum of Marina Blue is 365 nanometers and the emission maximum occurs at 460 nanometers. Cdc6 is expressed in actively replicating cells to function during eukaryotic replication initiation, and is essential for DNA synthesis. The narrow bandpass filter in the BV-1A combination does not efficiently excite Marina Blue, and produces only a weak signal. In addition, the specimen was simultaneously stained for F-actin with Alexa Fluor 488 conjugated to phalloidin, and for mitochondria with MitoTracker Red CMXRos. Note the presence of substantial signal levels from the red (MitoTracker) and green (Alexa Fluor 488) probes.