Rat Skeletal Muscle CDC6

CFP Bandpass Emission (Narrow Bandwidth Excitation) Blue-Violet Set

Fluorescence emission intensity from a culture of rat skeletal muscle tissue cells (L6 cell line; myoblasts) that were immunofluorescently labeled with primary anti-cdc6 (human) mouse monoclonal antibodies followed by goat anti-mouse Fab fragments conjugated to Marina Blue. The ultraviolet absorption maximum of Marina Blue is 365 nanometers and the emission maximum occurs at 460 nanometers. Cdc6 is expressed in actively replicating cells to function during eukaryotic replication initiation, and is essential for DNA synthesis. The narrow bandpass filter in the CFP combination does not efficiently excite the ultraviolet light absorbing Marina Blue, and produces only a weak signal. In addition, the specimen was simultaneously stained for F-actin with Alexa Fluor 488 conjugated to phalloidin, and for mitochondria with MitoTracker Red CMXRos. Note the absence of signal from the red (MitoTracker) fluorophore, but the presence of low actin fluorescence levels from the green (Alexa Fluor 488) probe.