Rat Skeletal Muscle Tubulin

CFP Bandpass Emission (Narrow Bandwidth Excitation) Blue-Violet Set

Fluorescence emission intensity from a culture of rat skeletal muscle tissue cells (L6 cell line; myoblasts) that were immunofluorescently labeled with primary anti-bovine alpha-tubulin mouse monoclonal antibodies followed by goat anti-mouse Fab fragments conjugated to Alexa Fluor 430. The visible light absorption maximum of Alexa Fluor 430 is 431 nanometers and the emission maximum occurs at 541 nanometers. Although the intracellular microtubule network is heavily stained using this protocol, the bandpass filter in the CFP combination does not pass a large amount of fluorescence emission from Alexa Fluor 430, and produces only a weak signal that is concentrated near the nucleus. In addition, the specimen was simultaneously stained for F-actin with Alexa Fluor 488 conjugated to phalloidin, and for mitochondria with MitoTracker Red CMXRos. Note the absence of signal from the red (MitoTracker) and green (Alexa Fluor 488) fluorophores.