Rat Thoracic Aorta CDC6
BV-2A Longpass Emission (Medium Bandwidth Excitation) Blue-Violet Set
Fluorescence emission intensity from a culture of rat thoracic aorta (muscle) cells that were immunofluorescently labeled with primary anti-cdc6 (human) protein monoclonal antibodies (mouse) followed by goat anti-mouse Fab fragments conjugated to Alexa Fluor 430. The visible light absorption maximum of Alexa Fluor 430 is 431 nanometers and the emission maximum occurs at 541 nanometers. Cdc6 is expressed in actively replicating cells to function during eukaryotic replication initiation, and is essential for DNA synthesis. The specimen was simultaneously stained for F-actin with Alexa Fluor 488 conjugated to phalloidin, and for mitochondria with MitoTracker Red CMXRos. Note the cyan fluorescence scattered throughout the cytoplasm due to Alexa Fluor 430 and the presence of substantial signal levels from the red (MitoTracker) and green (Alexa Fluor 488) probes.