Rat Thoracic Aorta Cell Intermediate Filaments

G-2A Longpass Emission (Wide Bandwidth Excitation) Green Set

Fluorescence emission produced from a culture of rat thoracic aorta (muscle) cells that were immunofluorescently labeled with primary anti-desmin (an intermediate filament protein) mouse monoclonal antibodies followed by goat anti-mouse Fab fragments conjugated to Alexa Fluor 546. The visible light absorption maximum of Alexa Fluor 546 is 556 nanometers and the emission maximum occurs at 573 nanometers. In addition, the specimen was simultaneously stained for F-actin with Alexa Fluor 350 (blue) conjugated to phalloidin, and for DNA with SYTOX Green. Note the absence of signal from both the blue and green fluorophores, but the high level of orange-red fluorescence intensity from the labeled desmin. The longpass emission G-1A filter combination displays a higher degree of background signal (red in the image above) than do the related Nikon bandpass emission filter sets, G-2E/C and Cy3.