Rat Thoracic Aorta Cellular Mitochondria
CFP Bandpass Emission (Narrow Bandwidth Excitation) Blue-Violet Set
Fluorescence emission intensity from a culture of rat thoracic aorta (muscle) cells that were immunofluorescently labeled with primary anti-oxphos complex V inhibitor protein monoclonal antibodies (mouse) followed by goat anti-mouse Fab fragments conjugated to Pacific Blue. The visible light absorption maximum of Pacific Blue is 410 nanometers and the emission maximum occurs at 455 nanometers. In addition, the specimen was simultaneously stained for F-actin with Alexa Fluor 488 (green) conjugated to phalloidin, and for DNA with SYTOX Orange. Note the absence of signal from both the orange and green fluorophores (SYTOX localized in the nucleus would appear green under these observation conditions). In many cases, SYTOX Orange stains a variety of cytoplasmic elements in addition to DNA, although this is not evident in the image.