Rat Thoracic Aorta Cellular Mitochondria
V-2A Longpass Emission (Medium Bandwidth Excitation) Violet Set
Fluorescence emission intensity from a culture of rat thoracic aorta (muscle) cells that were immunofluorescently labeled with primary anti-desmin (an intermediate filament protein) mouse monoclonal antibodies followed by goat anti-mouse Fab fragments conjugated to Pacific Blue. The visible light absorption maximum of Pacific Blue is 410 nanometers and the emission maximum occurs at 455 nanometers. In addition, the specimen was simultaneously stained for F-actin with Alexa Fluor 568 (red) conjugated to phalloidin, and for DNA with SYTOX Green. Note the presence of signal from both the red and green fluorophores. In many cases, SYTOX Green stains a variety of cytoplasmic elements in addition to DNA, as is evident in the image.