Sagittal Rat Brain Section Stained for Myelin Binding Protein, GFAP, and Nuclear DNA

Myelin was targeted in the sagittal rat brain section presented above with mouse anti-myelin BP antibodies followed by goat anti-mouse secondary antibodies conjugated to Alexa Fluor 488. In addition, glial fibrillary acidic protein was targeted in the tissue section by treating the specimen with rabbit anti-GFAP antibodies visualized with goat anti-rabbit secondary antibodies conjugated to Alexa Fluor 568 (pseudocolored blue). Nuclear DNA was counterstained with Hoechst 33342 (pseudocolored red). Images were recorded in grayscale with a 12-bit digital camera coupled to a Nikon Eclipse 80i microscope equipped with bandpass emission fluorescence filter optical blocks. During the processing stage, individual image channels were pseudocolored with RGB values corresponding to each of the fluorophore emission spectral profiles unless otherwise noted above.