Sheep Bladder Tissue
The filamentous actin present in a sample of sheep bladder tissue (illustrated above) was targeted with Alexa Fluor 350 conjugated to a phallotoxin, phalloidin. In addition, the specimen was stained with Oregon Green 488 conjugated to wheat germ agglutinin, a lectin that selectively binds N-acetylglucosamine and N-acetylneuraminic (sialic acid) residues. SYTOX Orange was employed to stain nucleic acids. Images were recorded in grayscale with a 12-bit digital camera coupled to either a Nikon E-600 or Eclipse 80i microscope equipped with bandpass emission fluorescence filter optical blocks. During the processing stage, individual image channels were pseudocolored with RGB values corresponding to each of the fluorophore emission spectral profiles.