Sheep Tongue Tissue
Phalloidin is a phallotoxin isolated from the highly toxic Amanita phalloides mushroom that binds polymerized actin (F-actin). Alexa Fluor 350 conjugated to phalloidin was utilized to target filamentous actin in the sheep tongue tissue sample featured in the digital image above. Oregon Green 488 conjugated to the lectin wheat germ agglutinin and the nucleic acid stain SYTOX Orange were also employed to label the specimen. Images were recorded in grayscale with a 12-bit digital camera coupled to either a Nikon E-600 or Eclipse 80i microscope equipped with bandpass emission fluorescence filter optical blocks. During the processing stage, individual image channels were pseudocolored with RGB values corresponding to each of the fluorophore emission spectral profiles.