extinction coefficient

As commonly used in the context of fluorescence, it is a measure of how well a fluorophore absorbs an incident photon. This determines a fluorophore's overall brightness together with its quantum yield.

Synonyms: molar absorption coefficient , mass extinction coefficient , attenuation coefficient

See also: brightness (fluorophore) , quantum yield , fluorescence , fluorophore

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Imaging Fluorescent Proteins

Filter requirements, photobleaching, objective choice, highlighters, and multicolor imaging.