halo

An artifact common to phase contrast microscopy of large phase objects, where dark objects will appear to have a bright border and bright objects will appear to have a dark border. It is caused by weakly diffracted light traveling through the objective phase ring.

Synonyms: halo effect

See also: phase contrast microscopy , apodized phase contrast

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Apodized Phase Contrast

Explore how specimen size affects the angle of diffracted light rays that pass through apodized phase plates.