modulation contrast

A label-free brightfield contrast technique using illumination through a slit aperture (positioned in an aperture plane in the illumination optics) and detection through an intensity modulator (in a conjugate aperture plane in the detection optics). It enables visualization of phase gradients.

Synonyms: Hoffman modulation contrast

See also: Nikon advanced modulation contrast , label-free

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Specialized Microscope Objectives

Phase contrast, DIC, fluorescence, darkfield, Hoffman, and reflected light.