Autofluorescence in Microscopy Literature References

Autofluorescence is a naturally-occurring phenomenon that is observed in many plant and animal tissues, but can also be introduced by fixatives. The emission spectrum of autofluorescence is usually very broad when compared to the spectra of synthetic dyes, quantum dots, or fluorescent proteins, and is therefore often difficult to separate from that produced by fluorescently labeled tags using traditional filtering methodology.

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