Bimolecular Fluorescence Complementation Literature References

Bimolecular fluorescence complementation (BiFC) is a technique that enables direct visualization of protein interactions in living cells. The methodology is based on the association between two non-fluorescent fragments of a fluorescent protein that begin to emit fluorescence when brought into close proximity by fusion partners that are capable of interacting. A number of protein interactions have been investigated using BiFC in many different cell types and organisms.

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Bimolecular Fluorescence Complementation