FRAP Literature References

The mobility of a fluorescent protein fusion can be assessed using a technique known as fluorescence recovery after photobleaching (FRAP). In practice, the fluorescently labeled molecules in a small region of the cell are irreversibly photobleached using high laser power, followed by monitoring the subsequent movement of the surrounding non-bleached fluorescent molecules into the photobleached region using low laser power. Fluorescent protein fusions are the tags of choice for use in FRAP because they can be bleached without detectable damage to the cell.