Fluorescent Speckle Microscopy Literature References
Fluorescent speckle microscopy (FSM) is a technique that is useful for the analysis of macromolecular dynamics in living cells. Fluorescent speckles are formed by the random association of fluorophores with a macromolecular structure and were originally discovered in microtubules. The methodology has since been expanded to study a wide variety of other cytoskeletal (and associated) proteins.
Recommended Literature
- Danuser, G. and Waterman-Storer, C. M. Quantitative fluorescent speckle microscopy of cytoskeleton dynamics. Annual Review of Biophysics and Biomolecular Structure 35: 361-389 (2006).
- Salmon, W. C., Adams, M. C. and Waterman-Storer, C. M. Dual wavelength fluorescent speckle microscopy reveals coupling of microtubule and actin movements in migrating cells. Journal of Cell Biology 158: 31-37 (2002).
- Waterman-Storer, C. M. and Danuser, G. New directions for fluorescent speckle microscopy. Current Biology 12: r633-r640 (2002).
- Waterman-Storer, C. M. and Salmon, E. D. How microtubules get fluorescent speckles. Biophysical Journal 75: 2059-2069 (1998).
Additional Literature Sources
- Jurado, C., Haserick, J. R. and Lee, J. Slipping or gripping? Fluorescent speckle microscopy in fish keratocytes reveals two different mechanisms for generating a retrograde flow of actin. Molecular Biology of the Cell 16: 507-518 (2005).
- Ponti, A., Machacek, M., Gupton, S. L., Waterman-Storer, C. M. and Danuser, G. Two distinct actin networks drive the protrusion of migrating cells. Science 305: 1782-1786 (2004).
- Ponti, A., Matov, A., Adams, M., Gupton, S., Waterman-Storer, C. M. and Danuser, G. Periodic patterns of actin turnover in lamellipodia and lamellae of migrating epithelial cells analyzed by quantitative fluorescent speckle microscopy. Biophysical Journal 89: 3456-3469 (2005).
- Ponti, A., Vallotton, P., Salmon, W. C., Waterman-Storer, C. M. and Danuser, G. Computational analysis of F-actin turnover in cortical actin meshworks using fluorescent speckle microscopy. Biophysical Journal 84: 3336-3352 (2003).
- Vallotton, P., Danuser, G., Bohnet, S., Meister, J. J. and Verkhovsky, A. B. Tracking retrograde flow in keratocytes: News from the front. Molecular Biology of the Cell 16: 1223-1231 (2005).
- Vallotton, P., Gupton, S. L., Waterman-Storer, C. M. and Danuser, G. Simultaneous mapping of filamentous actin flow and turnover in migrating cells by quantitative fluorescent speckle microscopy. Proceedings of the National Academy of Sciences (USA) 101: 9660-9665 (2004).
- Watanabe, N. and Mitchision, T. J. Single-molecule speckle analysis of actin filament turnover in lamellipodia. Science 295: 1083-1086 (2002).
- Waterman-Storer, C. M., Desai, A., Bulinski, J. C. and Salmon, E. D. Fluorescent speckle microscopy, a method to visualize the dynamics of protein assemblies in living cells.Current Biology 8: 1227-1230 (1998).
- Zhou, F. Q., Waterman-Storer, C. M. and Cohan, C. S. Focal loss of actin bundles causes microtubule redistribution and growth cone turning. Journal of Cell Biology 157: 839-849 (2002).
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