Co-Localization Microscopy Literature References

Co-localization describes the presence of two or more different molecules in very close spatial positions within a specimen, which is often visualized using confocal microscopy with synthetic fluorophores or fluorescent proteins. The technique attempts to determine whether there is the potential for interaction between the two fluorophores, but investigators should be cautious about obtaining images at the proper spatial (three dimensional) resolution in order to avoid artifacts. Co-localization is analyzed through the use of scatterplots.

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Fluorophore Co-Localization