FRET Literature References

Limitations in determination of the spatial proximity of protein molecules can be overcome by applying Förster (or Fluorescence) Resonance Energy Transfer (FRET) microscopy techniques. FRET occurs between two appropriately positioned fluorophores only when the distance separating them is 8 to 10 nanometers or less. Thus, FRET is well-suited to the investigation of protein interactions that occur between two molecules positioned within several nanometers of each other. Over the past ten years, FRET approaches have gained popularity due to the rise in applications requiring genetically targeting of specific proteins and peptides using fusions to green fluorescent protein (GFP) and its mutated derivatives.