Live Cell Imaging using Superresolution Literature References
The investigation of interacting protein dynamics is an excellent candidate for unraveling complex biological concepts using superresolution microscopy. In proof-of-concept studies using point-spread function engineering and single-molecule superresolution imaging, most of the specimens have been fixed cells and tissues where dynamic studies are impossible. A limited number of experiments are now beginning to address cellular dynamics using superresolution microscopy techniques.
Recommended Literature
- Biteen, J. S. and Moerner, W. E. Single-molecule and superresolution imaging in live bacteria cells. Cold Spring Harbor Laboratory Press 2: a000448-8 (2010).
- Biteen, J. S., Thompson, M. A., Tselentis, N. K., Bowman, G. R., Shapiro, L. and Moerner, W. E.Super-resolution imaging in live Caulobacter crescentus cells using photoswitchable EYFP. Nature Methods 5: 947-949 (2008).
- Hess, S. T., Gould, T. J., Gudheti, M. V., Maas, S. A., Mills, K. D. and Zimmerberg, J. Dynamic clustered distribution of hemagglutinin resolved at 40 nm in living cell membranes discriminates between raft theories. Proceedings of the National Academy of Sciences (USA) 104: 17370-17375 (2007).
- Hirvonen, L. M., Wicker, K., Mandula, O. and Heintzmann, R. Structured illumination microscopy of a living cell. European Biophysics Journal 38: 807-812 (2009).
- Kner, P., Chhun, B. B., Griffis, E. R., Winoto, L. and Gustafsson, M. G. L. Super-resolution video microscopy of live cells by structured illumination. Nature Methods 6: 339-342 (2009).
- Niu, L. and Yu, J. Investigating intracellular dynamics of FtsZ cytoskeleton with photoactivation single-molecule tracking. Biophysical Journal 95: 2009-2016 (2008).
- Manley, S., Gillette, J. M. and Lippincott-Schwartz, J. High-density mapping of single-molecule trajectories with photoactivated localization microscopy. Nature Methods 5: 155-157 (2008).
- Saffarian, S. and Kirchhausen, T. Differential evanescence nanometry: Live-cell fluorescence measurements with 10-nm axial resolution on the plasma membrane.Biophysical Journal 94: 2333-2342 (2008).
- Shroff, H., Galbraith, C. G., Galbraith, J. A. and Betzig, E. Live-cell photoactivated localization microscopy of nanoscale adhesion dynamics. Nature Methods 5: 417-423 (2008).
- Westphal, V., Rizzoli, S. O., Lauterbach, M. A., Kamin, D., Jahn, R. and Hell, S.W. Video-rate far-field optical nanoscopy dissects synaptic vesicle movement. Science 320: 246-249 (2008).
Additional Literature Sources
- Chen, I., Howarth, M, Lin, W. and Ting, A. Y. Site-specific labeling of cell surface proteins with biophysical probes using biotin ligase. Nature Methods 2: 99-104 (2005).
- Guignet, E. G., Hovius, R. and Vogel, H. Reversible site-selective labeling of membrane proteins in live cells. Nature Biotechnology 22: 440-444 (2004).
- Gustafsson, M. G. L. Super-resolution light microscopy goes live. Nature Methods 5: 385-387 (2008).
- Knemeyer, J., Herten, D. P. and Sauer, M. Detection and identification of single molecules in living cells using spectrally resolved fluorescence lifetime imaging microscopy. Analytical Chemistry 75: 2147-2153 (2003).
- Kunik, D., Aramendia, P. F. and Martinez, O. E. Single photon fluorescent microlithography for live-cell imaging. Microscopy Research and Technique 73: 20-26 (2010).
- Lin, J. Y., Huang, R. P., Tsai, P. S. and Lee, C. H. Wide-field super-resolution optical sectioning microscopy using a single spatial light modulator. Journal of Optics A: Pure and Applied Optics 11: 015301-6 (2009).
- Muzzey, D. and van Oudenaarden, A. Quantitative time-lapse fluorescence microscopy in single cells. Annual Review of Cell and Developmental Biology 25: 301-327 (2009).
- Nagerl, U. V., Willig, K. I., Hein, B., Hell, S. W. and Bonhoeffer, T. Live-cell imaging of dendritic spines by STED microscopy. Proceedings of the National Academy of Sciences (USA)105: 18982-18987 (2008).
- Neurohr, G. and Gerlich, D. W. Assays for mitotic chromosome condensation in live yeast and mammalian cells. Chromosome Research 17: 145-154 (2009).
- Pinaud, F. and Dahan, M. Zooming into live cells. Science 320: 187-188 (2008).
- Subach, F. V., Patterson, G. H., Manley, S., Gillette, J. M., Lippincott-Schwartz, J. and Verkhusha, V. V. Photoactivatable mCherry for high-resolution two-color fluorescence microscopy. Nature Methods 6: 153-159 (2009).
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