Photoactivated Localization Microscopy (PALM) Literature References
Similar in concept to STORM, photoactivated localization microscopy utilizes optical highlighter fluorescent proteins to stochastically switch on a sub-population of molecules for sequential single-molecule readout. The fundamental principle behind PALM and related methodology is that the activated state of a photoswitchable molecule must lead to the consecutive emission of sufficient photons to enable precise localization before it enters a dark state or becomes deactivated by photobleaching.
Recommended Literature
- Betzig, E., Patterson, G. H., Sougrat, R., Lindwasser, O. W., Olenych, S., Bonifacino, J. S., Davidson, M. W., Lippincott-Schwartz, J. and Hess, H. F. Imaging intracellular fluorescent proteins at nanometer resolution. Science 313: 1642-1645 (2006).
- Gould, T. J., Gunewardene, M. S., Gudheti, M. V., Verkhusha, V. V., Yin, S., Gosse, J. A. and Hess, S. T. Nanoscale imaging of molecular position and anisotropies. Nature Methods 5:1027-1030 (2008).
- Gould, T. J., Verkhusha, V. V. and Hess, S. T. Imaging biological structures with fluorescence photoactivation localization microscopy. Nature Protocols 4: 291-308 (2009).
- Henriques, R. and Mhlanga, M. M. PALM and STORM: What hides beyond the Rayleigh limit? Biotechnology Journal 4: 846-857 (2009).
- Hess, S. T., Giriajan, T. P. K. and Mason, M. D. Ultra-high resolution imaging by fluorescence photoactivation localization microscopy. Biophysical Journal 91: 4258-4272 (2006).
- Manley, S., Gillette, J. M. and Lippincott-Schwartz, J. Single-particle tracking photoactivated localization microscopy for mapping single-molecule dynamics. Methods in Enzymology475: 109-120 (2010).
- Manley, S., Gillette, J. M., Patterson, G. H., Shroff, H., Hess, H. F., Betzig, E. and Lippincott-Schwartz, J. High-density mapping of single-molecule trajectories with photoactivated localization microscopy. Nature Methods 5: 155-157 (2008).
- Patterson, G. H., Davidson, M., Manley, S. and Lippincott-Schwartz, J. Superresolution imaging using single-molecule localization. Annual Review of Physical Chemistry 61: 345-367 (2010).
- Shroff, H., Galbraith, C. G., Galbraith, J. A. and Betzig, E. Live-cell photoactivated localization microscopy of nanoscale adhesion dynamics. Nature Methods 5: 417-423 (2008).
- Shroff, H., Galbraith, C. G., Galbraith, J. A., White, H., Gillette, J., Olenych, S., Davidson, M. W. and Betzig, E. Dual-color superresolution imaging of genetically expressed probes within individual adhesion complexes. Proceedings of the National Academy of Sciences (USA)104: 20308-20313 (2007).
- Shroff, H., White, H. and Betzig, E. Photoactivation localization microscopy (PALM) of adhesion complexes. Current Protocols in Cell Biology 41: 4.21.27 (2008).
- Subach, F. V., Patterson, G. H., Manley, S., Gillette, J. M., Lippincott-Schwartz, J. and Verkhusha, V. V. Photoactivatable mCherry for high-resolution two-color fluorescence microscopy. Nature Methods 6: 153-159 (2009).
Additional Literature Sources
- Baddeley, D., Batram, C., Weiland, Y., Cremer, C. and Birk, U. J. Nanostructure analysis using spatially modulated illumination microscopy. Nature Protocols 2: 2640-2646 (2007).
- Flors, C., Hotta, J. I., Uji-i, H., Dedecker, P., Ando, R., Mizuno, H., Miyawaki, A. and Hofkens, J.A stroboscopic approach for fast photoactivation-localization microscopy with Dronpa mutants. Journal of the American Chemical Society 129: 13970-13977 (2007).
- Greenfield, D., McEvoy, A. L., Shroff, H., Crooks, G. E., Wingreen, N. S., Betzig, E. and Liphardt, J. Self-organization of the Escherichia coli chemotaxis network imaged with super-resolution light microscopy. PLoS Biology 7: e1000137-11 (2009).
- Hess, S. T., Gould, T. J., Gudheti, M. V., Maas, S. A., Mills, K. D. and Zimmerberg, J. Dynamic clustered distribution of hemagglutinin resolved at 40 nm in living cell membranes discriminates between raft theories. Proceedings of the National Academy of Sciences (USA) 104: 17370-17375 (2007).
- Juette, M. F., Gould, T. J., Lessard, M. D., Mlodzianoski, M. J., Nagpure, B. S., Bennett, B. T., Hess, S. T. and Bewersdorf, J. Three-dimensional sub-100 nm resolution fluorescence microscopy of thick samples. Nature Methods 5: 527-529 (2008).
- McKinney, S. A., Murphy, C. S. , Hazelwood, K. L., Davidson, M. W. and Looger, L. L. A bright and photostable photoconvertible fluorescent protein. Nature Methods 6: 131-133 (2009).
- Mizuno, H., Dedecker, P., Ando, R., Fukano, T., Hofkens, J. and Miyawaki, A. Higher resolution in localization microscopy by slower switching of a photochromic protein. Photochemical and Photobiological Sciences 9: 239-248 (2010).
- Patterson, G. H. , Betzig, E., Lippincott-Schwartz, J. and Hess, H. F. Developing Photoactivated Localization Microscopy (PALM). IEEE International Symposium on Biomedical Imaging: From Nano to Macro: 940-943 (2007).
- Peng, W. PALM reading. Nature Methods 6: 243 (2009).
- Shtengel, G., Galbraith, J. A., Galbraith, C. G., Lippincott-Schwartz, J., Gillette, J. M., Manley, S., Sougrat, R., Waterman, C. M., Kanchanawong, P., Davidson, M. W., Fetter, R. D. and Hess, H. F.Interferometric fluorescent super-resolution microscopy resolves 3D cellular ultrastructure. Proceedings of the National Academy of Sciences (USA) 106: 3125-3130 (2009)
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