PALM with Independently Running Acquisition Literature References
A modification of photoactivated localization microscopy (PALM) overcomes several inherent limitations, such as the often high level of background signal and long image acquisition times. Termed PALMIRA (PALM with Independently Running Acquisition), the methodology is useful with photoswitchable fluorescent proteins and synthetic fluorophores. In many cases image acquisition is accelerated over 100-fold, thus reducing recording time to between 2 and 10 minutes. In addition, the large reduction in background signal potentially enabling imaging deep within the interior of intact cells.
Recommended Literature
- Dedecker, P., Flors, C., Hotta, J. I., Uji-i, H. and Hofkens, J. 3D nanoscopy: Bringing biological nanostructures into sharp focus. Angewandte Chemie International Edition 46: 8330-8332 (2007).
- Egner, A., Geisler, C., von Middendorff, C., Bock, H., Wenzel, D., Medda, R., Andresen, M., Stiel, A. C., Jakobs, S., Eggeling, C., Schonle, A. and Hell, S. W. Fluorescence nanoscopy in whole cells by asynchronous localization of photoswitching emitters. Biophysical Journal 93:3285-3290 (2007).
- Geisler, C., Schonle, A., von Middendorff, C., Bock, H., Eggeling, C., Egner, A. and Hell, S. W. Resolution of wavelength/10 in fluorescence microscopy using fast single molecule photo-switching. Applied Physics A: Materials Science & Processing 88: 223-226 (2007).
- Bock, H., Geisler, C., Wurm, C. A., von Middendorff, C., Jakobs, S., Schonle, A., Egner, A., Hell, S. W. and Eggeling, C. Two-color far-field fluorescence nanoscopy based on photoswitchable emitters. Applied Physics B: Lasers and Optics 88: 161-165 (2007).
- Folling, J., Belov, V., Kunetsky, R., Medda, R., Schonle, A., Egner, A., Eggeling, C., Bossi, M. and Hell, S. W. Photochromic rhodamines provide nanoscopy with optical sectioning.Angewandte Chemie International Edition 46: 6266-6270 (2007).
- Folling, J., Belov, V., Riedel, D., Schonle, A., Egner, A., Eggeling, C., Bossi, M. and Hell, S. W. Fluorescence nanoscopy with optical sectioning by two-photon induced molecular switching using continuous-wave lasers. ChemPhysChem 9: 321-326 (2008).
- Flors, C., Hotta, J. I., Uji-i, H., Dedecker, P., Ando, R., Mizuno, H., Miyawaki, A. and Hofkens, J. A stroboscopic approach for fast photoactivation-localization microscopy with Dronpa mutants. Journal of the American Chemical Society 129: 13970-13977 (2007).
- Juette, M. F., Gould, T. J., Lessard, M. D., Mlodzianoski, M. J., Nagpure, B. S., Bennett, B. T., Hess, S. T. and Bewersdorf, J. Three-dimensional sub-100nm resolution fluorescence microscopy of thick samples. Nature Methods 5: 527-529 (2008).
- Mlodzianoski, M. J., Juette, M. F., Beane, G. L. and Bewersdorf, J. Experimental characterization of 3D localization techniques for particle-tracking and super-resolution microscopy. Optics Express 17: 8264-8277 (2009).
- Stiel, A. C., Andresen, M., Bock, H., Hilbert, M., Schilde, J., Schonle, A., Eggeling, C., Egner, A., Hell, S. W. and Jakobs, S. Generation of monomeric reversibly switchable red fluorescent proteins for far-field fluorescence nanoscopy. Biophysical Journal 95: 2989-2997 (2008).
- Testa, I., Schonle, A., von Middendorff, C., Geisler, C., Medda, R., Wurm, C. A., Stiel, A. C., Jakobs, S., Bossi, M., Eggeling, R., Hell, S. W. and Egner, A. Nanoscale separation of molecular species based on their rotational mobility. Optics Express 16: 21093-21104 (2008).
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