PALM with Independently Running Acquisition Literature References

A modification of photoactivated localization microscopy (PALM) overcomes several inherent limitations, such as the often high level of background signal and long image acquisition times. Termed PALMIRA (PALM with Independently Running Acquisition), the methodology is useful with photoswitchable fluorescent proteins and synthetic fluorophores. In many cases image acquisition is accelerated over 100-fold, thus reducing recording time to between 2 and 10 minutes. In addition, the large reduction in background signal potentially enabling imaging deep within the interior of intact cells.

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PALMIRA Superresolution Imaging