Saturated Structured Illumination Microscopy Literature References
Saturated pattern excitation microscopy (SPEM) and saturated structured illumination microscopy (SSIM) are non-linear methods that deplete the fluorophore ground state by saturated excitation to generate a sinusoidal emission pattern that is recorded on an area-array CCD detector. The saturated excitation illumination produces narrow line-shaped dark regions in the zero nodes that are surrounded by high levels of fluorescence signal to generate a negative imprint of the features being imaged. Information about the specimen is retrieved mathematically during post-acquisition processing.
Recommended Literature
- Gustafsson, M. G. L. Nonlinear structured-illumination microscopy: Wide-field fluorescence imaging with theoretically unlimited resolution. Proceedings of the National Academy of Sciences (USA) 102: 13081-13086 (2005).
- Enderlein, J. Breaking the diffraction limit with dynamic saturation optical microscopy.Applied Physics Letters 87: 094105-3 (2005).
- Fujita, K., Kobayashi, M., Kawano, S., Yamanaka, M. and Kawata, S. High-resolution confocal microscopy by saturated excitation of fluorescence. Physical Review Letters 99: 228105-4 (2007).
- Haeberle, O., Simon, B Saturated structured confocal microscopy with theoretically unlimited resolution. Optics Communications 282: 3657-3664 (2009).
- Heintzmann, R. Saturated patterned excitation microscopy with two-dimensional excitation patterns. Micron 34: 283-291 (2003).
- Heintzmann, R., Jovin, T. M. and Cremer, C. Saturated patterned excitation microscopy-a concept for optical resolution improvement. Journal of the Optical Society of America A19: 1599-1609 (2002).
- Humpolickova, J., Benda, A. and Enderlein, J. Optical saturation as a versatile tool to enhance resolution in confocal microscopy. Biophysical Journal 97: 2623-2629 (2009).
- Stemmer, A., Beck, M. and Fiolka, R. Widefield fluorescence microscopy with extended resolution. Histochemistry and Cell Biology 130: 807-817 (2008).
- Wei, F. and Liu, Z. Plasmonic structured illumination microscopy. Nano Letters 10: 2531-2536 (2010).
- Yamanaka, M., Kawano, S., Fujita, K., Smith, N. I. and Kawata, S. Beyond the diffraction-limit biological imaging by saturated excitation microscopy. Journal of Biomedical Optics 13:050507-3 (2008).
Additional Literature Sources
- Frohn, J. T., Knapp, H. F. and Stemmer, A. True optical resolution beyond the Rayleigh limit achieved by standing wave illumination. Proceedings of the National Academy of Sciences (USA) 97: 7232-7236 (2000).
- Gustafsson, M. G. L. Surpassing the lateral resolution limit by a factor of two using structured illumination microscopy. Journal of Microscopy 198: 82-87 (2000).
- Heintzmann, R. and Gustafsson, M. G. L. Subdiffraction resolution in continuous samples.Nature Photonics 3: 362-364 (2009).
- Heintzmann, R., Jovin, T. M. and Cremer, C. Saturated patterned excitation microscopy-a concept for optical resolution improvement. Journal of the Optical Society of America A19: 1599-1609 (2002).
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