Single Molecule Imaging Literature References
Single molecule fluorescence spectroscopy and microscopy are powerful methods that are used to examine a multitude of phenomena using advanced techniques such as total internal reflection (TIRF) and superresolution imaging. For example, when fused to peptides and proteins that target kinesin and myosin biomolecular motors, fluorescent proteins have proven useful as probes to unravel the behavior of individual motor assemblies. Single molecule imaging combined with TIRF has been used to create superresolution images with a resolution of 10 to 20 nanometers.
Recommended Literature
- Peterman, E. J. G., Sosa, H. and Moerner, W. E. Single-molecule fluorescence spectroscopy and microscopy of biomolecular motors. Annual Review of Physical Chemistry 55: 79-96 (2004).
- Ha, T. Single-molecule fluorescence methods for the study of nucleic acids. Current Opinion in Structural Biology 11: 287-292 (2001).
- Haustein, E. and Schwille, P. Single-molecule spectroscopic methods. Current Opinion in Structural Biology 14: 531-540 (2004).
- Weiss, S. Measuring conformational dynamics of biomolecules by single molecule fluorescence spectroscopy. Nature Structural and Molecular Biology 7: 724-729 (2000).
- Ishijima, A. and Yanagida, T., Single molecule nanobioscience. Trends in Biochemical Sciences 26: 438-444 (2001).
- Roy, R., Hohng, S. and Ha, T. A practical guide to single-molecule FRET. Nature Methods 5:507-516 (2008).
- Rhoades, E., Gussakovsky, E. and Haran, G. Watching proteins fold one molecule at a time.Proceedings of the National Academy of Sciences (USA) 100: 3197-3202 (2003).
- Moerner, W. E. and Fromm, D. P. Methods of single-molecule fluorescence spectroscopy and microscopy. Review of Scientific Instruments 74: 3597-3619 (2003).
- Weiss, S. Fluorescence spectroscopy of single biomolecules. Science 283: 1676-1683 (1999).
- Kural, C., Kim, H., Syed, S., Goshima, G., Gelfand, V. I. and Selvin, P. R. Kinesin and dynein move a peroxisome in vivo: a tug-of-war or coordinated movement? Science 308: 1469-1472 (2005).
- Lu, H. P., Xun, L. and Xie, X. S. Single-molecule enzymatic dynamics. Science 282: 1877-1882 (1998).
- Walter, N. G., Huang, C. Y., Manzo, A. J. and Sobhy, M. A. Do-it-yourself guide: how to use the modern single-molecule toolkit. Nature Methods 5: 475-489 (2008).
- Blum, C. and Subramaniam, V. Single-molecule spectroscopy of fluorescent proteins.Analytical and Bioanalytical Chemistry 393: 527-541 (2009).
- Churchman, L. S., Okten, Z., Rock, R. S., Dawson, J. F. and Spudich, J. A. Single molecule high resolution colocalization of Cy3 and Cy5 attached to macromolecules measures intramolecular distances through time. Proceedings of the National Academy of Sciences (USA) 102: 1419-1423 (2005).
- Garcia-Parajo, M. F., Veerman, J. A., Bouwhuis, R., Valleee, R. and Van Hulst, N. F. Optical probing of single fluorescent molecules and proteins. ChemPhysChem 2: 347-360 (2001).
- Gordon, M. P., Ha, T. and Selvin, P. R. Single-molecule high-resolution imaging with photobleaching. Proceedings of the National Academy of Sciences (USA) 101: 6462-6465 (2004).
- Ha, T. Single-molecule fluorescence resonance energy transfer. Methods 25: 78-86 (2001).
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