Single Molecule Imaging Literature References

Single molecule fluorescence spectroscopy and microscopy are powerful methods that are used to examine a multitude of phenomena using advanced techniques such as total internal reflection (TIRF) and superresolution imaging. For example, when fused to peptides and proteins that target kinesin and myosin biomolecular motors, fluorescent proteins have proven useful as probes to unravel the behavior of individual motor assemblies. Single molecule imaging combined with TIRF has been used to create superresolution images with a resolution of 10 to 20 nanometers.

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Single Molecule Fluorescence Microscopy