Single-Molecule Localization for Superresolution Literature References

Single fluorescent molecules can be isolated from one another on the basis of one or more distinguishing optical characteristics and used as a basis for imaging using superresolution techniques with specimens having densely packed fluorophores. In this case, each fluorescent emitter can be localized to a much higher precision by determining the center of the point-spread function by applying a statistical fit of the ideal Gaussian to its measured photon distribution.

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Single-Molecule Localization