Spectral Imaging Microscopy Literature References

Spectral imaging and linear unmixing is a powerful tool that is ideal for separating the signals in two or more fluorophores having closely overlapping emission spectra. The technique has proven useful for removing unwanted autofluorescence, enhancing co-localization analysis in confocal microscopy, and for unraveling intermolecular FRET interactions between fluorescent proteins and synthetic fluorophores. Spectral imaging can be implemented in widefield microscopy, but is more commonly applied in specialized confocal microscopes equipped with detectors designed to maximize signal collection.

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Spectral Imaging and Linear Unmixing