Spinning Disk Microscopy Literature References

Spinning disk confocal microscopy utilizes multiple pinholes or slits to project a series of 1000 or more parallel excitation light beams onto the specimen in a multiplexed pattern that is subsequently detected after fluorescence emission passes through the same pinholes or slits. The technique is highly useful for high speed imaging of living cells expressing fluorescent proteins or stained with membrane-permeant synthetic dyes. Photobleaching and phototoxicity are reduced with spinning disk microscopy, but a tighter light budget requires very sensitive camera systems for optimum signal detection.